Empty Affinity Chromatography (AC) Column Kits

Packing AC Columns

The section describes a manual method

packing AC columns.

1. Pretreating Frits

Place the top and bottom frits into deionized water or 20% ethanol to remove the bubbles existing in the frit.

2. Loading Bottom Frit

Add a little deionized water or 20% ethanol to the bottom of a column. Then, push the bottom frit to the bottom by plunger and cap the column outlet.

3. Adding Resin

Transfer the required volume of resin by a plastic pipette to the column. Allow the resin to settle and fill the column.

4. Loading Top Frit

Push the top frit horizontally to the top of the column by plunger.

5. Sealing and Storage

Fill the column with an appropriate solvent. Take care and avoid the bubbles. Seal the upper cap and tighten the bottom cap.

Ordering Information

Cat.#	Description	Qty.
006001	Empty AC Column Kits for 1 mL Columns	10Sets/Box
006002	Empty AC Column Kits for 3 mL Columns	10Sets/Box
006003	Empty AC Column Kits for 6 mL Columns	10Sets/Box
006004	Empty AC Column Kits for 12 mL Columns	10Sets/Box
006005	Empty AC Column Kits for 30 mL Columns	5Sets/Box
006006	Empty AC Column Kits for 60 mL Columns	5Sets/Box
006009-1	Empty AC Column Kits for 150 mL Columns	5Sets/Box
006008	Empty AC Column Kits for 300 mL Columns	3Sets/Box
006007	Empty AC Columns Kits for1mL Luer-Inlet Columns	10Sets/Box