| Application |
For the cultivation of Saccharomyces cerevisiae and Pichia pastoris |
| Preparation |
Suspend 50.0 g in 1 L of distilled water. Heat with frequent agitation and boil to completely dissolve the powder. Autoclave at 121° C for 15 minutes. Yeast has a relatively wide tolerance to pH, so the pH of YPD broth is typically not adjusted. If required, the pH may be adjusted to 6.5 before sterilization by autoclaving |
| Principle |
Peptone and yeast extract provide essential nitrogen sources, vitamins, and growth factors; D-glucose serves as the carbon source |
| Formula |
Formula(1L) |
Amount(g/L) |
| Yeast Extract |
10 |
| Peptone |
20 |
| Dextrose (D-Glucose) |
20 |
| Shelf Life |
3 Years |