| Application |
Provides a nitrogen source, macronutrients, micronutrients, and all essential vitamins required for yeast growth |
| Preparation |
Suspend 6.7 g in 1 L of distilled water, warm slightly to dissolve, Sterilize by filtration or autoclaving at 121 ° C for 15 minutes. Grams per liter may be adjusted. or formula supplemented to obtain desired performance Yeast has a relatively wide tolerance to pH, so the pH of YNB is typically not adjusted. |
| Principle |
YNB provides the essential nutrients, vitamins, and inorganic salts necessary for yeast growth. It does not contain histidine, methionine, tryptophan, or a carbon source, and therefore requires supplementation according to experimental needs. |
| Formula |
Formula(1L) |
Amount(g/L) |
| Ammonium sulfate |
5.0g |
| Monopotassium phosphate |
1.0g |
| Magnesium Sulfate |
0.5g |
| Sodium Chloride |
0.1g |
| Calcium D-Pantothenate |
400.0μg |
| Folic Acid |
2.0μg |
| Inositol |
2.0mg |
| Niacin |
400.0μg |
| p-Aminobenzoic Acid |
200.0μg |
| Pyridoxine HCl (Vitamin B6) |
400.0μg |
| Riboflavin (Vitamin B2) |
200.0μg |
| Thiamine HCl (Vitamin B1) |
400.0μg |
| Boric Acid |
500.0μg |
| Copper Sulfate |
40.0μg |
| Potassium Iodide |
100.0μg |
| Ferric Chloride |
200.0μg |
| Manganese Sulfate |
400.0μg |
| Sodium Molybdate |
200.0μg |
| Zinc Sulfate |
400.0μg |
| Shelf Life |
3 Years |