CommaSil® C4 300 Å HPLC Column
CommaSil®C4 300 Å HPLC Column employs a unique bonding process and fully capping technology to achieve a high degree of coverage of the bonded phase, effectively eliminating the influence of residual silica hydroxyl groups on the analysis. The 300 Å pore size is especially suitable for separating peptide and protein samples, demonstrating strong retention capabilities for both hydrophobic and polar compounds. Additionally, the unique double end-capping technology minimize the impact of residual silica hydroxyls and ensuring excellent peak shapes for the separation of basic and strongly polar compounds. Overall, CommaSil® C4 300 Å HPLC Column is highly suitable for separating peptide and protein samples, maintaining sharp peak shapes while offering excellent selectivity for both hydrophobic and polar compounds.
Details
Determination of LactoferrinChromatographic column: CommaSil® C4 300 Å, (4.6×250 mm, 5um)
Cat. #: HC4536
Flow rate: 1.0 mL/min
Detector: UV detector
Wavelength: 280 nm
Injection volume: 50 μL
Mobile phase: 0.1% trifluoroacetic acid
Table 1. Gradient Elution Program
| Time (min) |
CAN (%) |
0.1% TFA (%) |
| 0 |
30 |
70 |
| 5 |
55 |
45 |
| 10 |
60 |
40 |
| 12 |
30 |
70 |
| 16 |
30 |
70 |
Ordering Information
| Particle size |
Specification |
Cat. # |
| 3μm |
30×2.1mm |
HC4114 |
| 50×2.1mm |
HC4214 |
| 100×2.1mm |
HC4314 |
| 150×2.1mm |
HC4414 |
| 30×3.0mm |
HC4124 |
| 50×3.0mm |
HC4224 |
| 100×3.0mm |
HC4324 |
| 150×3.0mm |
HC4424 |
| 250×3.0mm |
HC4524 |
| 30×4.6mm |
HC4134 |
| 50×4.6mm |
HC4234 |
| 100×4.6mm |
HC4334 |
| 150×4.6mm |
HC4434 |
| 250×4.6mm |
HC4534 |
| 3.5μm |
30×2.1mm |
HC4115 |
| 50×2.1mm |
HC4215 |
| 100×2.1mm |
HC4315 |
| 150×2.1mm |
HC4415 |
| 30×3.0mm |
HC4125 |
| 50×3.0mm |
HC4225 |
| 100×3.0mm |
HC4325 |
| 150×3.0mm |
HC4425 |
| 250×3.0mm |
HC4525 |
| 30×4.6mm |
HC4135 |
| 50×4.6mm |
HC4235 |
| 100×4.6mm |
HC4335 |
| 150×4.6mm |
HC4435 |
| 250×4.6mm |
HC4535 |
| 5μm |
30×2.1mm |
HC4116 |
| 50×2.1mm |
HC4216 |
| 100×2.1mm |
HC4316 |
| 150×2.1mm |
HC4416 |
| 30×3.0mm |
HC4126 |
| 50×3.0mm |
HC4226 |
| 100×3.0mm |
HC4326 |
| 150×3.0mm |
HC4426 |
| 250×3.0mm |
HC4526 |
| 30×4.6mm |
HC4136 |
| 50×4.6mm |
HC4236 |
| 100×4.6mm |
HC4336 |
| 150×4.6mm |
HC4436 |
| 250×4.6mm |
HC4536 |
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