

| Application | Minimal SD Base is a synthetic yeast basal medium that can be supplemented with specific DO Supplements to formulate SD-series auxotrophic selective media. | |
| Preparation | Suspend 26.7g of minimal SD base and 1.3g of DO supplement in 1L of distilled water, sterilize by filtration or autoclaving at 121 ° C for 15 minutes | |
| Principle | Minimal SD Base consists of YNB and dextrose, providing essential growth factors, inorganic salts, an inorganic nitrogen source, and a carbon source. Auxotrophic selection or reporter gene activation is achieved by supplementing with the appropriate DO Supplement. | |
| Formula | Formula(1L) | Amount(g/L) |
| YNB Powder | 6.7 | |
| Dextrose (D-Glucose) | 20 | |
| Agar(optional) | 20 | |
| Shelf Life | 3 Years | |
| Cat. # | Description | Qty |
| GF960F | Minimal SD Base | 267g/Bottle |