Nucleic Acid Hybridization Reagents

Biocomma nucleic acid hybridization reagents include PBS buffer and SSC buffer.
PBS buffer can effectively neutralize NaOH in gels, preventing subsequent high-salt transfer buffers (such as SSC) from losing effectiveness or causing nucleic acid damage due to excessively high pH.
SSC solution, as a saline buffer, is mainly used to neutralize the negative charges on the DNA phosphate backbone, reducing electrostatic repulsion between two single strands and thereby promoting hybridization.
When used as a wash buffer, the stringency of washing is regulated by controlling the ionic strength and temperature of the SSC solution.

Ordering Information

Cat.#	Description	Formula	Qty.	Carton Size
PBS-CZ	PBS Buffer（1×）	137 mM NaCl，2.7 mM KCl，10 mM Na2HPO4，1.8 mM KH2PO4	500mL/ bottle ,10 bottle / box	420x180x195mm
PBSC510A	PBS Buffer（10×）	1.37 mM NaCl，27 mM KCl，100 mM Na2HPO4，18 mM KH2PO4	500mL/ bottle ,10 bottle / box	420x180x195mm
SSC-500Z-1	SSC Buffer （1×）	15 mM Sodium Citrate, 150 mM NaCl，pH 7.0	500mL/ bottle ,10 bottle / box	420x180x195mm
SSC-520Z-1	SSC Buffer （20×）	300 mM Sodium Citrate, 3 M NaCl，pH 7.0	500mL/ bottle ,10 bottle / box	420x180x195mm