2×YT Broth

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Product Information

Application	2×YT Medium provides rich nutrient for high-density E. coli growth and phage propagation (e.g., M13 and other ssDNA phages).
Preparation	Suspend 31.0g in 1 L of purified water. Heat with frequent agitation and boil to completely dissolve the powder. Autoclave at 121 ℃ for 20 minutes.
Principle	Tryptone and yeast extract provide carbon sources, nitrogen sources, vitamins, and minerals. Sodium chloride maintains balanced osmotic pressure.
Formula	Formula(1L)	Amount（g/L）
	Tryptone	16
	Yeast Extract	10
	Sodium Chloride	5
	pH (25℃)	7.0 ±0.2

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