LB Broth (Miller)

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Product Information

Application	The media is used for the cultivation of Escherichia coli in molecular biology experiments. (Molecular Cloning: A Laboratory Manual 4th Edition). PH 7.0 is suitable for general bacterial culture, strain maintenance, and routine laboratory use.
Preparation	Suspend 25.0 g in 1 L of purified water. Heat with frequent agitation and boil to completely dissolve the powder.Autoclave at 121° C for 20 minutes.
Principle	Tryptone and Yeast Extract provide nitrogen sources, vitamins and growth factors. Sodium chloride maintains balanced osmotic pressure.
Formula	Formula(1L)	Amount（g/L）
	Tryptone	10
	Yeast Extract	5
	Sodium Chloride	10
	pH (25℃)	7.0 ±0.2

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