LB Agar (Miller), PH 7.5

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Product Information

Application	The media is used for the cultivation of Escherichia coli in molecular biology experiments. PH 7.5 is optimized for molecular cloning, plasmid preparation, high-density E. coli culture, and protein expression applications.
Preparation	Suspend 40.0 g in 1 L of purified water. Heat with frequent agitation and boil to completely dissolve the powder. Autoclave at 121° C for 20 minutes.
Principle	Tryptone and Yeast Extract provide nitrogen sources, vitamins and growth factors. Sodium chloride maintains balanced osmotic pressure. Agar is the coagulant of the medium.
Formula	Formula(1L)	Amount（g/L）
	Tryptone	10
	Yeast Extract	5
	Sodium Chloride	10
	Agar	15
	pH (25℃)	7.5 ±0.2

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