LB Broth (Lennox), PH 7.5

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Application	The media is used for the cultivation of Escherichia coli in molecular biology experiments. (Molecular Cloning: A Laboratory Manual 4th Edition). PH 7.5 is optimized for molecular cloning, plasmid preparation, high-density E. coli culture, and protein expression applications.
Preparation	Suspend 20.0 g in 1 L of purified water. Heat with frequent agitation and boil to completely dissolve the powder. Autoclave at 121° C for 20 minutes.
Principle	Tryptone and Yeast Extract provide nitrogen sources, vitamins and growth factors. Sodium chloride maintains balanced osmotic pressure.
Formula	Formula(1L)	Amount（g/L）
	Tryptone	10
	Yeast Extract	5
	Sodium Chloride	5
	pH (25℃)	7.5 ±0.2

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