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  • Empty Affinity Chromatography (AC)  Column Kits
  • Empty Affinity Chromatography (AC)  Column Kits

Empty Affinity Chromatography (AC) Column Kits

biocomma® Empty Affinity Chromatography (AC) Column Kits include empty tubes, frits, upper and bottom caps, plunger, syringe and connecting pipe for customers to pack their own media easily and handily.

Details

Packing AC Columns

The section describes a manual method

packing AC columns.

1. Pretreating Frits

Place the top and bottom frits into deionized water or 20% ethanol to remove the bubbles existing in the frit.

2. Loading Bottom Frit

Add a little deionized water or 20% ethanol to the bottom of a column. Then, push the bottom frit to the bottom by plunger and cap the column outlet.

3. Adding Resin

Transfer the required volume of resin by a plastic pipette to the column. Allow the resin to settle and fill the column.

4. Loading Top Frit

Push the top frit horizontally to the top of the column by plunger.

5. Sealing and Storage

Fill the column with an appropriate solvent. Take care and avoid the bubbles. Seal the upper cap and tighten the bottom cap.

Ordering Information

Cat.#    Description Qty.
006001    Empty AC Column Kits for 1 mL Columns 10Sets/Box
006002    Empty AC Column Kits for 3 mL Columns 10Sets/Box
006003    Empty AC Column Kits for 6 mL Columns 10Sets/Box
006004    Empty AC Column Kits for 12 mL Columns 10Sets/Box
006005    Empty AC Column Kits for 30 mL Columns 5Sets/Box
006006    Empty AC Column Kits for 60 mL Columns 5Sets/Box
006009-1    Empty AC Column Kits for 150 mL Columns 5Sets/Box
006008    Empty AC Column Kits for 300 mL Columns 3Sets/Box
006007    Empty AC Columns Kits for1mL Luer-Inlet Columns 10Sets/Box

 

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