CommaSil® C18 Universal HPLC Column

CommaSil® C18 HPLC Columns as the prime choice among general-purpose columns. Furthermore, its exceptional chromatographic performance, superb selectivity, and reliable reproducibility make it the preferred option for method development under non-extreme chromatographic conditions.

Features

● General purpose columns boasting excellent column-to-column and lot-to-lot reproducibility.
● Exceptional selectivity and versatility across a wide range of applications.
● Universally applicable to pharmaceutical, environmental, and food safety testing fields.
● Equivelent to Waters Symmetry C18 series.

L-Tryptophan
Chromatographic column: CommaSil® C18 (4.6×150 mm, 3. 5μm)
Cat. #: HC18435
Detector: UV 280 nm
Flow rate: 0.8 ml/min
Column temperature: 35 ℃
Mobile phase: 10 mM ammonium acetate solution (pH 4.0):
methanol = 90:10

                                                                 Chromatogram of L-Tryptophan

Olaquinol Metabolites
Chromatographic column: CommaSil® C18 (4.6 mm×250 mm, 5 μm)
Cat#: HC18536
Mobile phase: A: methanol, B: 1% aqueous formic acid solution
Elution mode: isocratic elution (A:B = 40:60)
Flow rate: 1.0 mL/min
Column temperature: 30 ℃
Injection volume: 40 μL
Detector: UV detector
Detection wavelength: 320 nm


                                                    Chromatogram of Olaquinol Metabolites

11 Synthetic Colorants
Chromatographic column: CommaSil® C18 (4.6 mm×250 mm, 5 μm)
Cat#: HC18536
Mobile Phase: A: 20 mmoL/L ammonium acetate, B: methanol
Flow rate: 1.0 mL/min
Column temperature: 30 ℃
Injection volume: 10 μL
Detector: UV detector
Detection wavelength: 400-800 nm
Table 1. Gradient Elution Program
 

Time (min)	A (%)	B (%)
0.01	90	10
12.0	65	35
19.0	55	45
22.5	50	50
23.0	45	55
24.0	35	65
34.0	35	65
35.0	90	10
42.0	90	10

                                                 Chromatograms of 11 Synthetic Colorants

415 nm:                                      520 nm:                                        610 nm:
1. Tartrazine;                               1. New Red;                                  1. Indigotine;
2. Quinoline Yellow 1;                 2. Amaranthus Red;                     2. Brilliant Blue 1;
3. Quinoline Yellow 2;                 3. Carmine Cochineal;                  3. Brilliant Blue 2.
4. Quinoline Yellow 3;                 4. Sunset Yellow;
5. Quinoline Yellow 4.                 5. Allura Red;
                                                    6. Carmoisine;
                                                    7. Erythrosine.

Cefazolin Sodium
Chromatographic column: CommaSil® C18 (4.6 mm×250 mm, 5 μm)
Cat#: HC18536
Mobile phase: A: phosphate buffer, B: acetonitrile
Flow rate: 1.2 mL/min
Column temperature: 45 ℃
Injection volume: 10 μL
Detector: UV-Vis detector
Detection wavelength: 254 nm
Table 1. Gradient Elution Program
 

Time (min)	A (%)	B (%)
0	98	2
2.0	98	2
4.0	85	15
10	60	40
11.5	35	65
12	35	65
15	98	2

                                                           Chromatogram of Cefazolin Sodium

Abamectin
Chromatographic column: CommaSil® C18 (4.6 mm×250 mm, 5 μm)
Cat#: HC18536
Mobile Phase: A: Methanol & Acetonitrile (50+50), B: Ultra-pure water
Elution mode: Gradient elution (A:B=90:10)
Flow rate: 1.0 mL/min
Column temperature: 40 ℃
Injection volume: 20 μL
Detector: UV detector


                                                             Chromatogram of Abamectin

6-Benzyladenine
Chromatographic column: CommaSil® C18 (4.6 mm×250 mm, 5 μm)
Cat#: HC18536
Mobile phase: A: methanol, B: 20 mmol/L ammonium acetate solution
Elution method: isocratic elution (A:B=50:50)
Flow rate: 1.0 mL/min
Column temperature: 30 ℃
Injection volume: 10 μL
Detector: UV detector
Detection wavelength: 267 nm


                                                             Chromatogram of 6-Benzyladenine

Benzo(a)pyrene
Chromatographic column: CommaSil® C18 (4.6 mm×250 mm, 5 μm)
Cat#: HC18536
Mobile phase: A: acetonitrile, B: water
Elution method: isocratic elution (A:B=88:12)
Flow rate: 1.0 mL/min
Column temperature: 25 ℃
Injection volume: 10 μL
Detector: FLD
Emission wavelength: 406 nm
Excitation wavelength: 384 nm


                                                               Chromatogram of Benzo(a)pyrene

Melamine
Chromatographic column: CommaSil® C18 (4.6 mm×250 mm, 5 μm)
Cat#: HC18536
Mobile phase: A: acetonitrile B: ion-pairing buffer reagent
Elution mode: isocratic elution (A:B=10:90)
Flow rate: 1.0 mL/min
Column temperature: 40 ℃
Injection volume: 20 μL
Detector: UV detector
Detection wavelength: 240 nm


                                                             Chromatogram of Melamine

Ochratoxin A
Chromatographic column: CommaSil® C18 (4.6 mm×250 mm, 5 μm)
Cat#: HC18536
Mobile phase: acetonitrile - water - glacial acetic acid (96+102+2)
Flow rate: 1.0 mL/min
Column temperature: 35 ℃
Injection volume: 50 μL
Detection wavelength: 460 nm
Emission wavelength: 460 nm
Excitation wavelength: 333nm


                                                              Chromatogram of Ochratoxin A

 

CommaSil® C18 HPLC Column Ordering Information

Particle size	Specification	Cat. #
3μm	30×2.1mm	HC18114
	50×2.1mm	HC18214
	100×2.1mm	HC18314
	150×2.1mm	HC18414
	30×3.0mm	HC18124
	50×3.0mm	HC18224
	100×3.0mm	HC18324
	150×3.0mm	HC18424
	250×3.0mm	HC18524
	30×4.6mm	HC18134
	50×4.6mm	HC18234
	100×4.6mm	HC18334
	150×4.6mm	HC18434
	250×4.6mm	HC18534
3.5μm	30×2.1mm	HC18115
	50×2.1mm	HC18215
	100×2.1mm	HC18315
	150×2.1mm	HC18415
	30×3.0mm	HC18125
	50×3.0mm	HC18225
	100×3.0mm	HC18325
	150×3.0mm	HC18425
	250×3.0mm	HC18525
	30×4.6mm	HC18135
	50×4.6mm	HC18235
	100×4.6mm	HC18335
	150×4.6mm	HC18435
	250×4.6mm	HC18535
5μm	30×2.1mm	HC18116
	50×2.1mm	HC18216
	100×2.1mm	HC18316
	150×2.1mm	HC18416
	30×3.0mm	HC18126
	50×3.0mm	HC18226
	100×3.0mm	HC18326
	150×3.0mm	HC18426
	250×3.0mm	HC18526
	30×4.6mm	HC18136
	50×4.6mm	HC18236
	100×4.6mm	HC18336
	150×4.6mm	HC18436
	250×4.6mm	HC18536