● Adopting a unique hybrid bare sphere structure, offering an expanded pH tolerance range.
● Triple bonding modification and unique end-capping technology ensure high column efficiency and excellent chromatographic peak symmetry.
● Strictly follow quality control standards to ensure the excellent lot-to-lot consistency of sorbent and columns.
● Equivelent to Waters BEH C18 series.
Malachite greenChromatographic column: CommaSil® C18-T (2.1 mm×100 mm,3 μm)
Cat#: HC18T314
Mobile phase: A: 0.1 % Formic acid water (Contains 5 mmol/L ammonium acetate), B: Acetonitrile
Flow rate: 0.3 mL/min
Column temperature: 40 ℃
Injection volume: 10 μL
Table 1. Gradient Elution Program
Time (min) |
A (%) |
B (%) |
0 |
40 |
60 |
1.1 |
40 |
60 |
3.5 |
10 |
90 |
4.5 |
10 |
90 |
5 |
40 |
60 |
6 |
40 |
60 |
Mass Spectrometry ConditionsSource of ion: HESI
Scan method: positive ion mode
Electrospray voltage: 3500 V
sheath gas pressure: 35 arb
Auxiliary gas pressure: 5 arb
Ion transfer tube: 380 ℃
Auxiliary air temperature: 320 ℃
Table 2. Targes and Characteristic Ions (*Quantitative Ions)
Mobile phase: A: water (0.1% formic acid) B: Methanol-Acetonitrile solution containing 0.1% formic acid (methanol: acetonitrile=2:8)
Targets |
Parent Ion |
Daughter Ion |
Malachite Green (MG) |
329 |
208, 313* |
Leuco Malachite Green (LMG) |
331 |
239*, 316 |
Malachite Green-D5 (MG-D5) |
334 |
318 |
Leuco Malachite Green-D6 (LMG-D6) |
337 |
322 |
Crystal Violet (CV) |
372 |
356*, 251 |
Leuco Malachite Green (LCV) |
374 |
238*, 358 |
![](/d/file/p/2024/07-03/ed121a9dee21a589ce73a3e5d5d03ded.jpg)
Figure 1. Ion Chromatogram of MG at 1 ppb
![](/d/file/p/2024/07-03/e076fc259e40aa545070aa46f6495416.jpg)
Figure 2. Ion Chromatogram of MG-D5 at 1 ppb
![](/d/file/p/2024/07-03/29c0bd53222045bacbe3ad52a9aa45e7.jpg)
Figure 3. Ion Chromatogram of LMG at 1 ppb
![](/d/file/p/2024/07-03/5adac1923a9a2129e0ed518903a62cf0.jpg)
Figure 4. Ion Chromatogram of LMG-D6 at 1 ppb
![](/d/file/p/2024/07-03/4602293a5e7d29c3c6b1d1fdcdc3f88e.jpg)
Figure 5. Ion Chromatogram of CV at 1 ppb
![](/d/file/p/2024/07-03/2f30e5005c1d4ee19d732638848c1b28.jpg)
Figure 6. Ion Chromatogram of LCV at 1 ppb
Aflatoxin B1Chromatographic column: CommaSil® C18-T (2.1 mm×100 mm, 3 μm)
Cat#: HC18T314
Mobile phase: A: Contains 5 mmol/L ammonium acetate,
B: 0.1% formic acid methanol
Flow rate: 0.3 mL/min
Column temperature: 40 ℃
Injection volume: 5 μL
Table 1. Gradient Elution Progra
Time (min) |
A (%) |
B (%) |
0 |
90 |
10 |
1.2 |
40 |
60 |
2.1 |
10 |
90 |
4.8 |
10 |
90 |
5 |
90 |
10 |
6 |
90 |
10 |
Mass Spectrometry Conditions
source of ion: HESI
Scan method: positive ion mode
Electrospray voltage: 3500 V
sheath gas pressure: 30 arb
Auxiliary gas pressure: 8 arb
Ion transfer tube: 300 ℃
Auxiliary air temperature: 350 ℃
Table 2. Targes and Characteristic Ions (*Quantitative Ions)
Targets |
Parent Ion |
Daughter Ion |
Aflatoxin B1 |
313.1 |
285*, 241.1 |
13C17-AFB1 |
330.1 |
255, 301* |
![](/d/file/p/2024/07-03/94396cc8e33019300dfe020399368fa1.jpg)
Figure 1. Ion Chromatograms of Aflatoxin B1
Determination of Pantoprazole Sodium Related SubstancesChromatographic column: CommaSil® C18-T 4.6×250mm, 5um
Cat. #: HC18T536
Detector: UV 289 nm
Flow rate: 1 ml/min
Column temperature: 40 ℃
Mobile phase
A: 0.01 mol/L dipotassium hydrogen phosphate solution (pH adjusted to 7.0 by phosphoric acid)
B: acetonitrile
Time (min) |
A (%) |
B (%) |
0 |
90 |
10 |
30 |
60 |
40 |
45 |
15 |
85 |
![](/d/file/p/2024/07-03/bea2123457c68dce97b9e250603df553.jpg)
In left to right order: Oxidative degradation impurities, Pantoprazole sodium
36 Veterinary Drug ResiduesInstrument: UPLC-MS/MS (Thermo Fisher TSQ Endura)
Chromatographic column: CommaSil® C18-T(2.1 mm×100 mm,3 μm)
Cat#: HC18T314
Mobile phase: A: Water (0.1% formic acid), B: Methanol:Acetonitrile = 2:8 (0.1% formic acid)
Flow rate: 0.3 mL/min
Column temperature: 35℃
Injection volume: 5 μL
Table 1. Gradient Elution Program
Time (min) |
A (%) |
B (%) |
0 |
98 |
2 |
3 |
90 |
10 |
8 |
65 |
35 |
10 |
20 |
80 |
11 |
5 |
95 |
12 |
98 |
2 |
14 |
98 |
2 |
Mass Spectrometry Conditions
Source of ion: HESI
Electrospray voltage: 3500 V
Sheath gas pressure: 40 arb
Auxiliary gas pressure: 2 arb
Ion transfer tube: 380 ℃
Auxiliary air temperature: 350 ℃
Table 2. Targets, Retention Times and Characteristic
Ions (*Quantifier Ion)
No. |
Targets |
Retain time |
Parent Ion |
Daughter Ion |
1 |
Acesulfonamide |
3.18 |
215.0 |
108.0、155.9* |
2 |
Sulfapyridine |
4.67 |
250.1 |
155.8*、183.9 |
3 |
Sulfadiazine |
3.79 |
251.1 |
92.1、155.9* |
4 |
Sulfamethoxazole |
7.67 |
254.0 |
108.1、155.9* |
5 |
Sulfathiazole |
4.62 |
256.0 |
155.9*、92.1 |
6 |
Flumequine |
8.59 |
262.0 |
201.9、244.1* |
7 |
Oxalinic acid |
10.07 |
262.0 |
215.9、244.1* |
8 |
Sulphamerazine |
5.03 |
265.1 |
155.8*、171.8 |
9 |
Sulfamethoxazole |
8.12 |
268.0 |
113.0、155.8* |
10 |
Sulfamethadiazole |
6.26 |
271.0 |
92.1、155.9* |
11 |
Benzoylsulfonamide |
8.59 |
277.0 |
107.9、155.9* |
12 |
Sulfadimetine |
3.22 |
279.1 |
124.0*、185.8 |
13 |
Sulfadimidine |
5.91 |
279.1 |
155.8、185.8* |
14 |
Sulfamethoxypyridazine |
6.21 |
281.0 |
155.8*、126.0 |
15 |
Sulfametoxydiazine |
6.36 |
281.0 |
155.9*、214.9 |
16 |
Sulfamonomethoxine |
7.10 |
281.0 |
155.9*, 214.9 |
17 |
Sulfachlorpyridazine |
7.19 |
285.0 |
92.1, 155.9* |
18 |
Sulfamethoxazine |
7.61 |
311.1 |
155.8*, 244.9 |
19 |
Sulfadimethoxypyrimidine |
9.05 |
311.1 |
155.8*, 244.8 |
20 |
Sulfaphenazole |
9.10 |
315.0 |
157.9*, 159.9 |
21 |
Norfloxacin |
5.34 |
320.1 |
233.0, 276.0* |
22 |
Enoxacin |
5.15 |
321.1 |
234.0, 303.0* |
23 |
Ciprofloxacin |
5.49 |
332.0 |
230.9, 288.0* |
24 |
Pefloxacin |
5.40 |
334.2 |
290.1*, 316.1 |
25 |
Lomefloxacin |
5.67 |
352.0 |
265.0*, 308.0 |
26 |
Danofloxacin |
5.76 |
358.2 |
314.1, 340.0* |
27 |
Enrofloxacin |
5.85 |
360.2 |
245.0, 316.0* |
28 |
Ofloxacin |
5.31 |
362.1 |
261.1, 318.1* |
29 |
Marbofloxacin |
4.96 |
363.1 |
320.0*, 342.0 |
30 |
Sarafloxacin |
6.28 |
386.2 |
299.1, 342.1* |
31 |
Difloxacin |
6.30 |
400.2 |
299.0, 356.1* |
32 |
Phthalate sulfathiazole |
8.11 |
404.0 |
148.9, 255.8 |
33 |
Doxycycline |
7.45 |
445.2 |
321.0, 428.0* |
34 |
Tetracycline |
5.55 |
445.2 |
410.0*, 427.0 |
35 |
Oxytetracycline |
5.33 |
461.2 |
426.0*, 443.0 |
36 |
Chlortetracycline |
6.92 |
479.1 |
444.0*, 462.0 |
Ion Chromatogram of Residues at 10 ng/mL
![](/d/file/p/2024/07-03/a49a15cd2331b06eede4bb8f61909b6e.jpg)
Acesulfonamide
![](/d/file/p/2024/07-03/9689f285f53baa087b2afd1feb717ea3.jpg)
Sulfadiazine
![](/d/file/p/2024/07-03/92d6ab0595d3b4edd60249e0aec84427.jpg)
Sulfathiazole
![](/d/file/p/2024/07-03/8e418fdca6f13af5805bb362e1955f45.jpg)
Enoxacin
![](/d/file/p/2024/07-03/130c5066824019dbf2556c99c73c1c8f.jpg)
Norfloxacin
![](/d/file/p/2024/07-03/70faedf94980dd877865f03f758d5d81.jpg)
Sulfadimetine
![](/d/file/p/2024/07-03/f69c9708508b07861b2d4d9b666847eb.jpg)
Marbofloxacin
![](/d/file/p/2024/07-03/990c5d3754f5f5f62c71b5c8a8b6502e.jpg)
Sulfapyridine
![](/d/file/p/2024/07-03/ca48111d3d01303ee66240072c7b48f3.jpg)
Ofloxacin
![](/d/file/p/2024/07-03/bac4d5b9f566ac5e5db667946170bd6b.jpg)
Sulphamerazine
![](/d/file/p/2024/07-03/66978f3fb111ee465f5c758e02f5d4ba.jpg)
Pefloxacin
![](/d/file/p/2024/07-03/e4c8ed7e8d0592b60a8528a12de701bf.jpg)
Ciprofloxacin
![](/d/file/p/2024/07-03/7bdedeb6758d2d236e19c120ec2df571.jpg)
Oxytetracycline
![](/d/file/p/2024/07-03/d656d87f748160b278369b5e8ba880b0.jpg)
Danofloxacin
![](/d/file/p/2024/07-03/650b79775f69e959a6e655be523766f6.jpg)
Sulfadimidine
![](/d/file/p/2024/07-03/43ee18f4b30af7464c2e7a7d339015ce.jpg)
Sarafloxacin
![](/d/file/p/2024/07-03/f5d63fdd13793cbbe8e4326982f8b439.jpg)
Sulfamethadiazole
![](/d/file/p/2024/07-03/62c84c5a697befee1e6a7acc2d05e3e5.jpg)
Chlortetracycline
![](/d/file/p/2024/07-03/b70ac00b096cbd5ee04874d597e825e0.jpg)
Lomefloxacin
![](/d/file/p/2024/07-03/3234e6d533736bdbac6e09a17e01aab1.jpg)
Enrofloxacin
![](/d/file/p/2024/07-03/cf8a0c1b8b3257acb1c81b6833a6a30b.jpg)
Difloxacin
![](/d/file/p/2024/07-03/ac4cf0486ef559e629d5366502e03eb0.jpg)
Sulfametoxydiazine
![](/d/file/p/2024/07-03/b00c97327ca647409399d16f3e810b69.jpg)
Sulfamonomethoxine
![](/d/file/p/2024/07-03/4427b73ab14fee814d07dcc545367394.jpg)
Sulfachlorpyridazine
![](/d/file/p/2024/07-03/976aa01a1ff41a67200cf450ec7c74bf.jpg)
Doxycycline
![](/d/file/p/2024/07-03/b5f16eb0103c0a75400ab2884c36c9a6.jpg)
Sulfamethoxazole
![](/d/file/p/2024/07-03/19ab8539f5ef6d8cf3d76464469708ba.jpg)
Sulfamethoxazole
![](/d/file/p/2024/07-03/a91175af7be4df562e1d3412b2362359.jpg)
Oxalinic acid
![](/d/file/p/2024/07-03/13f33a347df75b6511b86b8d5ba1675a.jpg)
Sulfaphenazole
![](/d/file/p/2024/07-03/81fa8a52ad61985d1e825e9c72774a62.jpg)
Tetracycline
![](/d/file/p/2024/07-03/2ff90bbafa976e66b60fe8818d1da4e3.jpg)
Sulfamethoxazine
![](/d/file/p/2024/07-03/e2603fe6700f15555c65f98e037c4c62.jpg)
Phthalate sulfathiazole
![](/d/file/p/2024/07-03/b1c490ac353e33534b01eb1bd8153ceb.jpg)
Benzoylsulfonamide
![](/d/file/p/2024/07-03/a623e3fbffcf92ebc4f174ac4f97d16f.jpg)
Sulfadimethoxypyrimidine
![](/d/file/p/2024/07-03/a649e649161f7852590a538eaf83fd25.jpg)
Flumequine
![](/d/file/p/2024/07-03/6c4908d947a3a85a16bfe6222f11ac21.jpg)
Sulfamethoxypyridazine
FumonisinsInstrument: UPLC-MS/MS (Thermo Fisher TSQ Endura)
Chromatographic column: CommaSil® C18-T (2.1x100 mm, 3 μm)
Cat#: HC18T314
Mobile phase: A: Water (0.1% formic acid), B: 0.1% formic acid in methanol
Flow rate: 0.3 mL/min
Column temperature: 35 ℃
Injection volume: 5 μL
Table 1. Gradient Elution Program
Time (min) |
A (%) |
B (%) |
0 |
60 |
40 |
1.5 |
60 |
40 |
6.5 |
5 |
95 |
7 |
5 |
95 |
7.5 |
60 |
40 |
10 |
60 |
40 |
Mass Spectrometry Conditions
Source of ion: HESI
Electrospray voltage: 3500 V
Sheath gas pressure: 40 arb
Auxiliary gas pressure: 2 arb
Ion transfer tube: 380 ℃
Auxiliary gas temperature: 350 ℃
Table 2. Targets and Characteristic Ions
No. |
Targets |
Parent Ion (m/z) |
Daughter Ion (m/z) |
1 |
Fumonisins B2 |
706.55 |
318.28 |
706.55 |
336.262 |
2 |
Fumonisins B1 |
722 |
334.25 |
722 |
352.26 |
![](/d/file/p/2024/07-03/82bdfea1e2d06f262cae7370567045c7.jpg)
Ion Chromatogram of Fumonisins B1
![](/d/file/p/2024/07-03/baff4687ed8a8ba5fb467271a591ff23.jpg)
Ion Chromatogram of Fumonisins B2
CommaSil® C18-T HPLC Column Ordering Information
Particle size |
Specification |
Cat. # |
1.7μm |
30×2.1mm |
HC18T111 |
50×2.1mm |
HC18T211 |
100×2.1mm |
HC18T311 |
150×2.1mm |
HC18T411 |
30×3.0mm |
HC18T121 |
50×3.0mm |
HC18T221 |
100×3.0mm |
HC18T321 |
150×3.0mm |
HC18T421 |
250×3.0mm |
HC18T521 |
2.5μm |
30×2.1mm |
HC18T113 |
50×2.1mm |
HC18T213 |
100×2.1mm |
HC18T313 |
150×2.1mm |
HC18T413 |
30×3.0mm |
HC18T123 |
50×3.0mm |
HC18T223 |
100×3.0mm |
HC18T323 |
150×3.0mm |
HC18T423 |
250×3.0mm |
HC18T523 |
30×4.6mm |
HC18T133 |
50×4.6mm |
HC18T233 |
100×4.6mm |
HC18T333 |
150×4.6mm |
HC18T433 |
250×4.6mm |
HC18T533 |
3μm |
30×2.1mm |
HC18T114 |
50×2.1mm |
HC18T214 |
100×2.1mm |
HC18T314 |
150×2.1mm |
HC18T414 |
30×3.0mm |
HC18T124 |
50×3.0mm |
HC18T224 |
100×3.0mm |
HC18T324 |
150×3.0mm |
HC18T424 |
250×3.0mm |
HC18T524 |
30×4.6mm |
HC18T134 |
50×4.6mm |
HC18T234 |
100×4.6mm |
HC18T334 |
150×4.6mm |
HC18T434 |
250×4.6mm |
HC18T534 |
3.5μm |
30×2.1mm |
HC18T115 |
50×2.1mm |
HC18T215 |
100×2.1mm |
HC18T315 |
150×2.1mm |
HC18T415 |
30×3.0mm |
HC18T125 |
50×3.0mm |
HC18T225 |
100×3.0mm |
HC18T325 |
150×3.0mm |
HC18T425 |
250×3.0mm |
HC18T525 |
30×4.6mm |
HC18T135 |
50×4.6mm |
HC18T235 |
100×4.6mm |
HC18T335 |
150×4.6mm |
HC18T435 |
250×4.6mm |
HC18T535 |
5μm |
30×2.1mm |
HC18T116 |
50×2.1mm |
HC18T216 |
100×2.1mm |
HC18T316 |
150×2.1mm |
HC18T416 |
30×3.0mm |
HC18T126 |
50×3.0mm |
HC18T226 |
100×3.0mm |
HC18T326 |
150×3.0mm |
HC18T426 |
250×3.0mm |
HC18T526 |
30×4.6mm |
HC18T136 |
50×4.6mm |
HC18T236 |
100×4.6mm |
HC18T336 |
150×4.6mm |
HC18T436 |
250×4.6mm |
HC18T536 |
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