CommaSil® C18-T Hybrid Silica HPLC Column

● Adopting a unique hybrid bare sphere structure, offering an expanded pH tolerance range.

● Triple bonding modification and unique end-capping technology ensure high column efficiency and excellent chromatographic peak symmetry.

● Strictly follow quality control standards to ensure the excellent lot-to-lot consistency of sorbent and columns.

● Equivelent to Waters BEH C18 series.

Malachite green
Chromatographic column: CommaSil® C18-T (2.1 mm×100 mm，3 μm）
Cat#: HC18T314
Mobile phase: A: 0.1 % Formic acid water (Contains 5 mmol/L ammonium acetate), B: Acetonitrile
Flow rate: 0.3 mL/min
Column temperature: 40 ℃
Injection volume: 10 μL
Table 1. Gradient Elution Program
 

Time (min)	A (%)	B (%)
0	40	60
1.1	40	60
3.5	10	90
4.5	10	90
5	40	60
6	40	60

Mass Spectrometry Conditions
Source of ion: HESI
Scan method: positive ion mode
Electrospray voltage: 3500 V
sheath gas pressure: 35 arb
Auxiliary gas pressure: 5 arb
Ion transfer tube: 380 ℃
Auxiliary air temperature: 320 ℃
Table 2. Targes and Characteristic Ions (*Quantitative Ions)

CommaSil® C18-H (2.1 mm×100 mm, 3 μm)

Mobile phase: A: water (0.1% formic acid) B: Methanol-Acetonitrile solution containing 0.1% formic acid (methanol: acetonitrile=2:8)
 

Targets	Parent Ion	Daughter Ion
Malachite Green (MG)	329	208, 313*
Leuco Malachite Green (LMG)	331	239*, 316
Malachite Green-D5 (MG-D5)	334	318
Leuco Malachite Green-D6 (LMG-D6)	337	322
Crystal Violet (CV)	372	356*, 251
Leuco Malachite Green (LCV)	374	238*, 358

Figure 1. Ion Chromatogram of MG at 1 ppb


Figure 2. Ion Chromatogram of MG-D5 at 1 ppb


Figure 3. Ion Chromatogram of LMG at 1 ppb


Figure 4. Ion Chromatogram of LMG-D6 at 1 ppb


Figure 5. Ion Chromatogram of CV at 1 ppb


Figure 6. Ion Chromatogram of LCV at 1 ppb

Aflatoxin B1
Chromatographic column: CommaSil® C18-T (2.1 mm×100 mm, 3 μm）
Cat#: HC18T314
Mobile phase: A: Contains 5 mmol/L ammonium acetate,
B: 0.1% formic acid methanol
Flow rate: 0.3 mL/min
Column temperature: 40 ℃
Injection volume: 5 μL
Table 1. Gradient Elution Progra
 

Time (min)	A (%)	B (%)
0	90	10
1.2	40	60
2.1	10	90
4.8	10	90
5	90	10
6	90	10

Mass Spectrometry Conditions
source of ion: HESI
Scan method: positive ion mode
Electrospray voltage: 3500 V
sheath gas pressure: 30 arb
Auxiliary gas pressure: 8 arb
Ion transfer tube: 300 ℃
Auxiliary air temperature: 350 ℃
Table 2. Targes and Characteristic Ions (*Quantitative Ions)

Targets	Parent Ion	Daughter Ion
Aflatoxin B1	313.1	285*, 241.1
13C17-AFB1	330.1	255, 301*

Figure 1. Ion Chromatograms of Aflatoxin B1

Determination of Pantoprazole Sodium Related Substances
Chromatographic column: CommaSil® C18-T 4.6×250mm, 5um
Cat. #: HC18T536
Detector: UV 289 nm
Flow rate: 1 ml/min
Column temperature: 40 ℃
Mobile phase
A: 0.01 mol/L dipotassium hydrogen phosphate solution (pH adjusted to 7.0 by phosphoric acid)
B: acetonitrile
 

Time (min)	A (%)	B (%)
0	90	10
30	60	40
45	15	85

In left to right order: Oxidative degradation impurities, Pantoprazole sodium

36 Veterinary Drug Residues
Instrument: UPLC-MS/MS (Thermo Fisher TSQ Endura)
Chromatographic column: CommaSil® C18-T（2.1 mm×100 mm,3 μm）
Cat#: HC18T314
Mobile phase: A: Water (0.1% formic acid), B: Methanol:Acetonitrile = 2:8 (0.1% formic acid)
Flow rate: 0.3 mL/min
Column temperature: 35℃
Injection volume: 5 μL
Table 1. Gradient Elution Program

Time (min)	A (%)	B (%)
0	98	2
3	90	10
8	65	35
10	20	80
11	5	95
12	98	2
14	98	2

Mass Spectrometry Conditions
Source of ion: HESI
Electrospray voltage: 3500 V
Sheath gas pressure: 40 arb
Auxiliary gas pressure: 2 arb
Ion transfer tube: 380 ℃
Auxiliary air temperature: 350 ℃
Table 2. Targets, Retention Times and Characteristic
Ions (*Quantifier Ion)
 

No.	Targets	Retain time	Parent Ion	Daughter Ion
1	Acesulfonamide	3.18	215.0	108.0、155.9*
2	Sulfapyridine	4.67	250.1	155.8*、183.9
3	Sulfadiazine	3.79	251.1	92.1、155.9*
4	Sulfamethoxazole	7.67	254.0	108.1、155.9*
5	Sulfathiazole	4.62	256.0	155.9*、92.1
6	Flumequine	8.59	262.0	201.9、244.1*
7	Oxalinic acid	10.07	262.0	215.9、244.1*
8	Sulphamerazine	5.03	265.1	155.8*、171.8
9	Sulfamethoxazole	8.12	268.0	113.0、155.8*
10	Sulfamethadiazole	6.26	271.0	92.1、155.9*
11	Benzoylsulfonamide	8.59	277.0	107.9、155.9*
12	Sulfadimetine	3.22	279.1	124.0*、185.8
13	Sulfadimidine	5.91	279.1	155.8、185.8*
14	Sulfamethoxypyridazine	6.21	281.0	155.8*、126.0
15	Sulfametoxydiazine	6.36	281.0	155.9*、214.9
16	Sulfamonomethoxine	7.10	281.0	155.9*, 214.9
17	Sulfachlorpyridazine	7.19	285.0	92.1, 155.9*
18	Sulfamethoxazine	7.61	311.1	155.8*, 244.9
19	Sulfadimethoxypyrimidine	9.05	311.1	155.8*, 244.8
20	Sulfaphenazole	9.10	315.0	157.9*, 159.9
21	Norfloxacin	5.34	320.1	233.0, 276.0*
22	Enoxacin	5.15	321.1	234.0, 303.0*
23	Ciprofloxacin	5.49	332.0	230.9, 288.0*
24	Pefloxacin	5.40	334.2	290.1*, 316.1
25	Lomefloxacin	5.67	352.0	265.0*, 308.0
26	Danofloxacin	5.76	358.2	314.1, 340.0*
27	Enrofloxacin	5.85	360.2	245.0, 316.0*
28	Ofloxacin	5.31	362.1	261.1, 318.1*
29	Marbofloxacin	4.96	363.1	320.0*, 342.0
30	Sarafloxacin	6.28	386.2	299.1, 342.1*
31	Difloxacin	6.30	400.2	299.0, 356.1*
32	Phthalate sulfathiazole	8.11	404.0	148.9, 255.8
33	Doxycycline	7.45	445.2	321.0, 428.0*
34	Tetracycline	5.55	445.2	410.0*, 427.0
35	Oxytetracycline	5.33	461.2	426.0*, 443.0
36	Chlortetracycline	6.92	479.1	444.0*, 462.0

Ion Chromatogram of Residues at 10 ng/mL


Acesulfonamide


Sulfadiazine


Sulfathiazole


Enoxacin


Norfloxacin


Sulfadimetine


Marbofloxacin


Sulfapyridine


Ofloxacin


Sulphamerazine


Pefloxacin


Ciprofloxacin


Oxytetracycline


Danofloxacin


Sulfadimidine


Sarafloxacin


Sulfamethadiazole


Chlortetracycline


Lomefloxacin


Enrofloxacin


Difloxacin


Sulfametoxydiazine


Sulfamonomethoxine


Sulfachlorpyridazine


Doxycycline


Sulfamethoxazole


Sulfamethoxazole


Oxalinic acid


Sulfaphenazole


Tetracycline


Sulfamethoxazine


Phthalate sulfathiazole


Benzoylsulfonamide


Sulfadimethoxypyrimidine


Flumequine


Sulfamethoxypyridazine

Fumonisins
Instrument: UPLC-MS/MS (Thermo Fisher TSQ Endura)
Chromatographic column: CommaSil® C18-T (2.1x100 mm, 3 μm）
Cat#: HC18T314
Mobile phase: A: Water (0.1% formic acid), B: 0.1% formic acid in methanol
Flow rate: 0.3 mL/min
Column temperature: 35 ℃
Injection volume: 5 μL
Table 1. Gradient Elution Program

Time (min)	A (%)	B (%)
0	60	40
1.5	60	40
6.5	5	95
7	5	95
7.5	60	40
10	60	40

Mass Spectrometry Conditions
Source of ion: HESI
Electrospray voltage: 3500 V
Sheath gas pressure: 40 arb
Auxiliary gas pressure: 2 arb
Ion transfer tube: 380 ℃
Auxiliary gas temperature: 350 ℃

Table 2. Targets and Characteristic Ions

No.	Targets	Parent Ion (m/z)	Daughter Ion (m/z)
1	Fumonisins B2	706.55	318.28
		706.55	336.262
2	Fumonisins B1	722	334.25
		722	352.26

Ion Chromatogram of Fumonisins B1


Ion Chromatogram of Fumonisins B2

CommaSil® C18-T HPLC Column Ordering Information

 

Particle size	Specification	Cat. #
1.7μm	30×2.1mm	HC18T111
	50×2.1mm	HC18T211
	100×2.1mm	HC18T311
	150×2.1mm	HC18T411
	30×3.0mm	HC18T121
	50×3.0mm	HC18T221
	100×3.0mm	HC18T321
	150×3.0mm	HC18T421
	250×3.0mm	HC18T521
2.5μm	30×2.1mm	HC18T113
	50×2.1mm	HC18T213
	100×2.1mm	HC18T313
	150×2.1mm	HC18T413
	30×3.0mm	HC18T123
	50×3.0mm	HC18T223
	100×3.0mm	HC18T323
	150×3.0mm	HC18T423
	250×3.0mm	HC18T523
	30×4.6mm	HC18T133
	50×4.6mm	HC18T233
	100×4.6mm	HC18T333
	150×4.6mm	HC18T433
	250×4.6mm	HC18T533
3μm	30×2.1mm	HC18T114
	50×2.1mm	HC18T214
	100×2.1mm	HC18T314
	150×2.1mm	HC18T414
	30×3.0mm	HC18T124
	50×3.0mm	HC18T224
	100×3.0mm	HC18T324
	150×3.0mm	HC18T424
	250×3.0mm	HC18T524
	30×4.6mm	HC18T134
	50×4.6mm	HC18T234
	100×4.6mm	HC18T334
	150×4.6mm	HC18T434
	250×4.6mm	HC18T534
3.5μm	30×2.1mm	HC18T115
	50×2.1mm	HC18T215
	100×2.1mm	HC18T315
	150×2.1mm	HC18T415
	30×3.0mm	HC18T125
	50×3.0mm	HC18T225
	100×3.0mm	HC18T325
	150×3.0mm	HC18T425
	250×3.0mm	HC18T525
	30×4.6mm	HC18T135
	50×4.6mm	HC18T235
	100×4.6mm	HC18T335
	150×4.6mm	HC18T435
	250×4.6mm	HC18T535
5μm	30×2.1mm	HC18T116
	50×2.1mm	HC18T216
	100×2.1mm	HC18T316
	150×2.1mm	HC18T416
	30×3.0mm	HC18T126
	50×3.0mm	HC18T226
	100×3.0mm	HC18T326
	150×3.0mm	HC18T426
	250×3.0mm	HC18T526
	30×4.6mm	HC18T136
	50×4.6mm	HC18T236
	100×4.6mm	HC18T336
	150×4.6mm	HC18T436
	250×4.6mm	HC18T536