After synthesis, oligonucleotides are released from solid support by treatment with alkaline solution. The resulting solution contains the anticipated full-length oligonucleotides as well as some failure sequences (error sequences), the amount of which depends on synthesis efficiency. In many applications such as PCR, these failure sequences compete with the full-length sequence, so they must be removed prior to use in order to improve yields of downstream applications.
The C18 desalting purification cartridge is packed with octadecyl (C18) bounded silica gel. The octadecyl groups are very hydrophobic and have strong retention for non-polar compounds. Thus DNA molecules can be retained while residual
salts pass through, endowing you with the power to obtain high-purity DNA products, then use in downstream applications, including PCR and sequencing.
biocomma® C18 desalting purification cartridges/plate suit for purification of oligo below 60mer.
||C18 Desalting Purification Cartridge,50 mg/1 mL
||C18 Desalting Purification Cartridge,500 mg/6 mL
||C18 Desalting Purification Plate,50mg/1.5mL/96well
||C18 Desalting Purification Plate,80mg/2mL/96well