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Modified Giolitti and Cantoni Broth Base

SKU: GF1678F

DESCRIPTION: Modified Giolitti and Cantoni Broth Base
APPLICATlON: For the enumeration in accordance to the MPN method and selective enrichment of Staphylococcus aureus.

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Product Information

PRODUCT NAME	Modified Giolitti and Cantoni Broth Base
PRODUCT CODE	GF1678F
DESCRIPTION	Modified Giolitti and Cantoni Broth Base
ACC	ISO 6888
APPLICATION	For the enumeration in accordance to the MPN method and selective enrichment of Staphylococcus aureus.
PREPARATION	1. Suspend 55.2 g in 1 L of purified water for the preparation of single strenght broth. Suspend 110.4 g in 1 L of purified water for the preparation of double strenght broth.
	2. Heat with frequent agitation and boil to completely dissolve the powder. Distribute 9 ml portions into tubes for the preparation of single strenght broth and 10 ml for the preparation of double strenght broth.Autoclave at121 'C for 15 minutes.
	3. Cool to 45-50 ℃ and aseptically add 0.1 ml and 0.2 ml per tube, of 1% potassium tellurite solution for single and double concentration respectively.

COMPOSITION	Ingredients	g/L
	Enzymatic Digest of Casein	10.0
	Meat Extract	5.0
	Yeast Extract	5.0
	Lithium Chloride	5.0
	Mannitol	20.0
	Sodium Chloride	5.0
	Glycine	1.2
	Sodium Pyruvate	3.0
	Tween 80	1.0
	Final pH (25℃)	6.9±0.2
STORAGE	Store in a dark and dry place at 2-25 ℃, tighten the bottle cap immediately after use.
SHELF LIFE	Shelf life of sealed product is 3 years.
PRECAUTIONS	1. NOT for clinical use.
	2. Do not use this product if it is lumpy.
	3. After use, all contaminated materials must be autoclaved at 121 °C for 30 minutes and then disposed of.

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