CommaSil® C18-P 100% Water Resistant HPLC Column Separation of Polar Compounds

Features

● Improves retention of polar compounds while maintaining moderate retention of moderately and strongly hydrophobic compounds.
● Tolerant to 100% aqueous mobile phases.
● Good separation reproducibility.
● Equivelent to Waters Atlantis C18 series.

Isolation of Emetine-Related Substances
Chromatographic column: CommaSil® C18-P (4.6×150 mm, 3 μm)
Cat. #: HC18P434
Detector: UV 280 nm
Flow rate: 0.8 mL/min
Column temperature: 37 ℃
Mobile phase
A: Buffer solution – acetonitrile (70:30)
B: Buffer solution – acetonitrile (50:50)
Buffer solution: weigh 2.5 g of sodium dodecyl sulphate and 3.9 g of anhydrous disodium hydrogen phosphate and dissolve in 900 mL of water. Adjust the pH to 3.4 by phosphoric acid, then, dilute to 1000 mL by water.

Time (min)	A (%)	B (%)
0	50	50
5	50	50
15	40	60
30	40	60
45	0	100
55	0	100
56	50	50
65	50	50

Method statement: regarding the separation of emastin from impurities C, E, F, and G, the CommaSil® C18-P HPLC Columns meet the separation requirements of the main components from all impurities. The separation of main emastin and impurity E could be greater than 1.5.

13 Organic Acids
Chromatographic column: CommaSil® C18-P (4.6 mm×250 mm, 5μm)
Cat#: HC18P536
Detector: UV 220 nm
Flow rate: 1 mL/min
Column temperature: 37 ℃
Mobile phase: 0.1% phosphoric acid aqueous solution


Liquid Chromatograms of 13 Organic Acids

Determination of Amoxicillin Clavulanate Potassium Content
Chromatographic columns: CommaSil® C18-P (250×4.6mm, 4.6μm)
Cat. #: HC18P536
Detector: UV 220 nm
Flow rate: 1 mL/min
Column temperature: 30 ℃
Mobile phase: 0.05 mol/L sodium dihydrogen phosphate solution - methanol = (95:5)


Chromatogram of Control Solution, 6.383 min: clavulanic acid, 11.244 min: amoxicillin

0.05 mol/L sodium dihydrogen phosphate solution: weigh 7.8 g of sodium dihydrogen phosphate, add dissolve in 900 ml of water. Adjust the pH to 4.4±0.1 by 10% phosphoric acid solution or sodium hydroxide test solution, then, dilute to 1000 mL by water.


Chromatogram System Suitability Solution, 6.424 min: clavulanic acid, 9.492 min: amoxicillin thiazole acid, 11.202 min: amoxicillin

CommaSil® C18-P HPLC Column Ordering Information

Particle size	Specification	Cat. #
3μm	30×2.1mm	HC18P114
	50×2.1mm	HC18P214
	100×2.1mm	HC18P314
	150×2.1mm	HC18P414
	30×3.0mm	HC18P124
	50×3.0mm	HC18P224
	100×3.0mm	HC18P324
	150×3.0mm	HC18P424
	250×3.0mm	HC18P524
	30×4.6mm	HC18P134
	50×4.6mm	HC18P234
	100×4.6mm	HC18P334
	150×4.6mm	HC18P434
	250×4.6mm	HC18P534
5μm	30×2.1mm	HC18P116
	50×2.1mm	HC18P216
	100×2.1mm	HC18P316
	150×2.1mm	HC18P416
	30×3.0mm	HC18P126
	50×3.0mm	HC18P226
	100×3.0mm	HC18P326
	150×3.0mm	HC18P426
	250×3.0mm	HC18P526
	30×4.6mm	HC18P136
	50×4.6mm	HC18P236
	100×4.6mm	HC18P336
	150×4.6mm	HC18P436
	250×4.6mm	HC18P536