Copure® HPLC Columns Specially for Mycotoxin

Ochratoxin A
Reference Method: GB 5009.96-2016
Column: Copure® HPLC ColumnsSpecially for Mycotoxin (4.6 mm×250 mm, 5 μm)
Part Number: HMY01
Mobile Phase: Acetonitrile-Water-Glacial
Acetic Acid (96+102+2)
Flow Rate: 1.0 mL/min
Column Temperature: 35℃
Injection Volume: 50 μL
Detection Wavelength: 460nm
Excitation Wavelength: 333nm


Ochratoxin A Liquid Chromatography

Fumonisins
Reference Method: GB 5009.240-2023
Instrument: UPLC-MS/MS (Thermo Fisher TSQ
Endura)
Column: Copure® HPLC Column for Mycotoxins
(2.1 mm×100 mm, 3 μm)
Part Number: HMY02
Mobile Phase: A: Water (0.1% Formic Acid)
B: 0.1% Formic Acid Methanol Solution
Elution Mode: Gradient Elution, see Table 1
Flow Rate: 0.3 mL/min
Column Temperature: 35℃
Injection Volume: 5 μL
Elution Program, Table 1
 

Time /min	A/%	B/%
0	60	40
1.5	60	40
6.5	5	95
7	5	95
7.5	60	40
10	60	40

Mass Spectrometry Conditions
Ion Source: HESI
Electrospray Voltage: 3500 V
Sheath Gas Pressure: 40 arb
Auxiliary Gas Pressure: 2 arb
Ion Transfer Tube: 380℃
Auxiliary Temperature: 350℃
See Table 2 for Ion Pair Information
Table 2 Ion Pair Information
 

No.	Item	Parent Ion (m/z)	Daughter Ion (m/z)
1	Fumonisin B2	706.55	318.28
		706.55	336.262
2	Fumonisin B1	722	334.25
		722	352.26

II. Extracted Ion Chromatograms of Fumonisins


Fumonisin B1


Fumonisin B2

Aflatoxin B1
Reference Method: GB 5009.22-2016
Column: Copure® HPLC Column for Mycotoxins (2.1
mm×100 mm, 3 μm)
Part Number: HMY02
Mobile Phase: A: 5 mmol/L Ammonium Acetate B:
0.1% Formic Acid Methanol
Flow Rate: 0.3 mL/min
Column Temperature: 40℃
Injection Volume: 5 μL
Elution Mode: Gradient Elution, see Table 1
Table 1: Gradient Elution Program
 

Time /min	A/%	B/%
0	90	10
1.2	40	60
2.1	10	90
4.8	10	90
5	90	10
6	90	10

Mass Spectrometry Conditions
Ion Source: HESI
Scan Mode: Positive Ion Scan
Electrospray Voltage: 3500 V
Sheath Gas Pressure: 30 arb
Auxiliary Gas Pressure: 8 arb
Ion Transfer Tube: 300℃
Auxiliary Temperature: 350℃
Table 2 Component Names, Retention Times, and
Characteristic Ions ( indicates Quantitative Ion)*
 

Component Name	Parent Ion	Daughter Ion
Aflatoxin B1	313.1	285*、241.1
13C17-AFB1	330.1	255、301*

Ion Chromatogram of Aflatoxin B1
 

Ordering Information

Cat.#	Specification	Qty.
HMY01	4.6mm×250mm，5μm	1 unit/box
HMY02	2.1mm×100mm，3μm	1 unit/box
HMY03	2.1mm×100mm，1.7μm	1 unit/box