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Biocomma Limited

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Copure® HPLC Columns Specially for Pesticide Residue

Copure® HPLC Columns Specially for Pesticide Residue utilize ultra-high purity silica with narrow particle size distribution, combined with mature and stable bonding technology and precise end-capping process, ensuring high column efficiency and a suitable pressure range. In pesticide residue analysis, it provides high sensitivity, excellent separation, and symmetrical peak shapes, thereby enhancing accuracy and reliability in detection.

Details

Instrument: UPLC-MS/MS (Thermo Fisher TSQ Endura)
Column: Copure® HPLC Columns Specially for Pesticide Residue (2.1 mm×100 mm, 3 μm)
Part Number: HNC01
Mobile Phase: A: Ammonium Formate-Formic Acid Water
(2mmol/L) B: Ammonium Formate-Formic Acid Methanol
(2mmol/L)
Flow Rate: 0.3 mL/min
Column Temperature: 40℃
Injection Volume: 2 μL
Elution Mode: Gradient Elution, see Table 1
Table 1: Gradient Elution Program

Time /min	A/%	B/%
0.00	97	3.0
1.00	97	3.0
1.50	85	15.0
2.50	50	50.0
18.0	30	70.0
23.0	2	98.0
25.0	2	98.0
25.1	97	3.0
27.0	97	3.0

4.2 Mass Spectrometry Conditions
Ion Source: HESI
Electrospray Voltage: Positive Ion Mode 3500 V, Negative Ion Mode 3500V
Sheath Gas Pressure: 35 arb
Auxiliary Gas Pressure: 3 arb
Ion Transfer Tube: 380℃
Auxiliary Temperature: 420℃