His Prepacked Columns

biocomma ^® CommaX ^TM His prepacked columns are packed with highly cross-linked Poly(methyl methacrylate) beads which are coated with a propriety hydrophilic layer, then grafted with IDA ligands which donate binding sites to Ni ²⁺ ions, forming an immobilized metal affinity chromatography (IMAC) medium for purifying recombinant proteins with polyhistidine tags.

Recombinant proteins with polyhistidine tags are retained due to the formation of complex compounds between their polyhistidine tags and immobilized Ni ²⁺ ions. Impurity proteins without polyhistidine tags are washed off. The target proteins are eluted with an imidazole buffer solution.

CommaX ^TM His prepacked columns are available in 1 mL and 5 mL sizes.

Features:

• Superior biocompatibility
• Uncompromised biological activities
• High purification yields
• Moderate experimental conditions

Applications:

• Purification of recombinant proteins with polyhistidine tags from various express systems, such as bacterial, insect and mammal cells.

Order information:

Note: CommaX ^TM His prepacked columns and Ni-IDA chromatographic medium should be stored at 2-8 °C.