CommaPrep® Nucleic Acid Purification Spin Columns are based on Biocomma’s proprietary spin column-based silica membrane purification technology. In addition to the spin column itself, each spin column comes with one outer tube (reservoir), one frit, one silica membrane and one fixing ring.

Under low pH and chaotropic conditions, nucleic acids specifically bind to silica membrane while polysaccharides and proteins pass through. Impurities are further removed by washing. Finally, under low-salt conditions, nucleic acids are desorbed and eluted from the membrane.

Nucleic acids purified by CommaPrep® Nucleic Acid Purification Spin Columns are suitable for various downstream routine applications, including restriction digestion, PCR amplification, sequencing, ligation and transformation.