Copure® HLB SPE Cartridges
Copure® HLB SPE Cartridges
Copure® HLB SPE Cartridges

Copure® HLB SPE Cartridges

Extracting apolar to moderately polar acidic, neutral and basic compounds

Copure ® HLB sorbent is composed of monodisperse N-vinylpyrrolidone-divinylbenzene copolymer resin particles, with specific mixture of hydrophilic hydrophobic groups, allowing for retention for a wide range of compounds with very high capacity.

HLB can be used as a general-purpose sorbent, especially for extracting analytes from complicated samples such as blood and urine.


  • General sorbent, suitable for wide application areas
  • Highly wettable, no worry of bed dryness, rare breakthrough
  • Highly recoveries, excellent reproducibility
  • 3 to 10 times higher adsorption capacity and loadability than C18-bounded silica gel
  • Stable from pH 1 to 14, compatible with common solvents


Surface area: 600 m 2/g
Particle size: 40 μm
Pore size: 300 Å


  • Determination of drugs, illicit drugs and their metabolites in blood, such as sertraline ibuprofen and heroin
  • Determination of residual antibiotics, catecholamines, and microcystins in foodstuff
  • Determination of veterinary drugs, pesticides and mycotoxin in milk products

Related methods:

  • GB/T 21315-2007   Determination of penicillins residues in foodstuffs of animal origin LC-MS/MS method
  • GB/T 21313-2007   Analysis of β-agonists in foods of animal origin by high performance liquid chromatography tandem mass spectrometry
  • GB 29685-2013    Determination of Lincomycin, Clindamycin and Spectinomycin residues in animal derived food by Gas Chromatography Mass Spectrometric method
  • GB 29682-2013   National Food Safety Standard Determination of Penicillins residues in aquatic products by High Performance Liquid Chromatographic method
  • NY/T 2067-2011   Determination of 13 sulfonylurea herbicides residues in soil by LC/MS/MS
  • SN/T 2050-2008   Determination of 14 beta-lactam antibiotic residues in foodstuffs of animal origin for export and import - LC-MS/MS method
  • SN/T 2654-2010   Determination of moroxydine residues in foodstuffs of animal origin for export and import - LC-MS/MS method
  • SN/T 2222-2008   Determination of glucocorticosteroids residues in foodstuffs of animal origin for import and export - LC-MS/MS method

Application: Determination of Quinolones in foods

Chromatographic Conditions

SPE Cartridge: Biocomma HLB, 200mg/3mL

System: Waters alliance 2690

Column: Welch Ultimate XB-C18 (4.6*250mm)

Mobile phase A: acetronile

Mobile phase B: water containing 0.1% formic acid

Flowrate: 1 mL/min

Column temperature: room temperature

Injection volume: 20 μL

Detector: UV at 220 nm


Step Time (min) A% B%
1 0 9 91
2 11 9 91
3 20 29 71
4 25 37 63
5 26 100 0
6 30 100 0
7 31 9 91
8 36 9 91


                                           Retention Time (min)

Peak Retention time (min) Analyte Recovery
1 21.508 Enoxacin 92.8%
2 22.820 Pefloxacin 82.4%
3 24.201 Danofloxacin 93.8%
4 24.754 Enrofloxacin 81.2%

The results suggest that recoveries of > 80% are obtained by using Biocomma HLB cartridges to extract the four  Quinolones in foods, meeting the criteria of China official method GB/T 21312-2007.

 Order information:

Product No. Description Format Qty.
COHLB130 Copure ® HLB SPE Cartridges 30mg/1mL 100/Box
COHLB1100 Copure ® HLB SPE Cartridges 100mg/1mL 100/Box
COHLB330 Copure ® HLB SPE Cartridges 30mg/3mL 50/Box
COHLB360 Copure ® HLB SPE Cartridges 60mg/3mL 50/Box
COHLB3200 Copure ® HLB SPE Cartridges 200mg/3mL 50/Box
COHLB3500 Copure ® HLB SPE Cartridges 500mg/3mL 50/Box
COHLB6150 Copure ® HLB SPE Cartridges 150mg/6mL 30/Box
COHLB6200 Copure ® HLB SPE Cartridges 200mg/6mL 30/Box
COHLB6500 Copure ® HLB SPE Cartridges 500mg/6mL 30/Box
COHLB12500 Copure ® HLB SPE Cartridges 500mg/12mL 20/Box
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